Journal: Cell reports

Article Title: Mining HIV controllers for broad and functional antibodies to recognize and eliminate HIV-infected cells

doi: 10.1016/j.celrep.2021.109167

Figure Lengend Snippet:

Article Snippet: Mouse Anti-Human C3 / C3b / iC3b Monoclonal Antibody, FITC Conjugated , CedarLane , CEDARLANE Cat# CL7632F; RRID:AB_10548984.

Techniques: Control, Virus, Recombinant, Reporter Gene Assay, Staining, Labeling, Software

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Costimulatory receptors in a teleost fish: typical CD28, elusive CTLA4.

doi: 10.4049/jimmunol.176.7.4191

Figure Lengend Snippet: FIGURE 8. Combined triggering of CD3 and huCD28/rbtCD28 in- duces IL-2 production. Jurkat CH7C17 transfectants expressing huCD28 wild-type (A14), huCD28/rbtCD28 receptor (2F5), huCD28/ rbtCTLA4 receptor (26H6) were incubated with ( ) or without (f) anti-human CD28 Ab for 30 min at 4°C. After washing, cells were deposited on anti-CD3-coated plates, and goat anti-mouse IgG1 Ab was added to cross-link human CD28 or chimeric receptors. Cells incubated only with goat anti-mouse IgG1 Ab were used as a control (). Su- pernatants were collected after 24 h at 37°C, and the IL-2 titer was determined by ELISA. IL-2 concentration is indicated in pg/10 ml. Asterisks indicate that the production of IL-2 by cells incubated using anti-human CD3-coated plates in the presence of anti-human CD28 Ab was significantly different from the production of IL-2 by cells solely plated on anti-human CD3-coated wells (two-tailed Mann-Whitney U test; , p 0.05, , p 0.01).

Article Snippet: Goat anti-mouse IgG1 Ab ( 1 chain specific; Southern Biotechnology Associates) was added (5 g/ ml) to cross-link human CD28.

Techniques: Expressing, Incubation, Control, Enzyme-linked Immunosorbent Assay, Concentration Assay, Two Tailed Test, MANN-WHITNEY

Journal: Scientific Reports

Article Title: In vitro complement activation via nucleocapsid and spike proteins of SARS-CoV-2 in COVID-19 patients

doi: 10.1038/s41598-025-20926-6

Figure Lengend Snippet: Comparison of the in vitro C3b and C4b complement depositions via spike (C3b(S) and C4b(S)) or nucleocapsid (C3b(N) and C4b(N)) recombinant proteins between patients with (Ab(+)) or without (Ab(-)) SARS-CoV-2-specific IgM and IgG antibodies from four different COVID-19 groups (CONV, HOSP, HOSP + O 2 and ICU). The in vitro C3b and C4b complement depositions were measured by our in-house complement deposition assays, where we used carrier free recombinant nucleocapsid or spike proteins and non-heat-inactivated patient sera. The p values for the pair-wise group comparisons on the violin plots were calculated by the Mann-Whitney tests. P values of significant differences are marked as p MW between without and with specific antibodies. Non-significant differences are marked as n.s.

Article Snippet: Mouse anti-human IgG1 Fc-HRP (HP6001) , SouthernBiotech , 9054-05.

Techniques: Comparison, In Vitro, Recombinant, MANN-WHITNEY

Journal: Scientific Reports

Article Title: In vitro complement activation via nucleocapsid and spike proteins of SARS-CoV-2 in COVID-19 patients

doi: 10.1038/s41598-025-20926-6

Figure Lengend Snippet: Comparison of the levels of SARS-CoV-2-specific IgM and IgG among four COVID-19 groups (CONV, HOSP, HOSP + O2, ICU) measured by Generic Assays CoV-2 IgM or IgG ( a ). The percentages of SARS-CoV-2-specific IgG or IgM positivity were shown also for four different COVID-19 groups. The numbers represent the mean percentages of SARS-CoV-2-specific IgM in blue or specific IgG in green circle sectors ( b ). The levels of total S- or N-specific IgG were quantified by in-house ELISA, normalized to positive and negative serum samples ( c ). The levels of S-specific and N-specific IgG1 ( d ) and IgG3 ( e ) subclasses were quantified by in-house ELISA calibrated with purified human IgG1 and IgG3. The p -values ( p KW ) were determined by Kruskal-Wallis test.

Article Snippet: Mouse anti-human IgG1 Fc-HRP (HP6001) , SouthernBiotech , 9054-05.

Techniques: Comparison, Enzyme-linked Immunosorbent Assay, Purification

Journal: Scientific Reports

Article Title: In vitro complement activation via nucleocapsid and spike proteins of SARS-CoV-2 in COVID-19 patients

doi: 10.1038/s41598-025-20926-6

Figure Lengend Snippet: The in vitro C3b and C4b complement depositions via spike (C3b(S) and C4b(S)) recombinant protein are plotted by the levels of S-specific IgG1 and IgG3 in CONV individuals and in the other three hospitalized COVID-19 groups (HOSP, HOSP + O 2 and ICU) ( a ). The in vitro C3b and C4b complement depositions via nucleocapsid (C3b(N) and C4b(N)) recombinant protein are plotted by the levels of N-specific IgG1 and IgG3 in CONV individuals and in the other three hospitalized COVID-19 groups (HOSP, HOSP + O 2 and ICU) ( b ).

Article Snippet: Mouse anti-human IgG1 Fc-HRP (HP6001) , SouthernBiotech , 9054-05.

Techniques: In Vitro, Recombinant

Journal: Scientific Reports

Article Title: In vitro complement activation via nucleocapsid and spike proteins of SARS-CoV-2 in COVID-19 patients

doi: 10.1038/s41598-025-20926-6

Figure Lengend Snippet: Comparison of the levels of SARS-CoV-2-specific IgM and IgG antibodies, and in vivo complement profiles in all COVID-19 patients between low and high in vitro C3b(N) complement depositions. The p values for the pair-wise group comparisons on the violin plots were calculated by the Mann-Whitney tests. Asterisks indicate p value < 0.0143 , considered significant results after 5% FDR correction using the Benjamini-Hochberg method.

Article Snippet: Mouse anti-human IgG1 Fc-HRP (HP6001) , SouthernBiotech , 9054-05.

Techniques: Comparison, In Vivo, In Vitro, MANN-WHITNEY

Journal: Cell reports

Article Title: The miR-17∼92 miRNAs promote plasma cell differentiation by suppressing SOCS3-mediated NIK degradation.

doi: 10.1016/j.celrep.2023.112968

Figure Lengend Snippet: Figure 6. SOCS3 promotes NIK ubiquitination and degradation (A) Cell lysates from WT iGCB cells cultured with 40LB plus IL-21 for 24 h were immunoprecipitated (IP) with anti-SOCS3 or IgG and analyzed by immunoblotting (IB) of SOCS3 and NIK. (B) Immunoblot analysis of NIK and GAPDH in WT and TKO iGCB cells cultured with 40LB cells plus IL-21 and treated with DMSO or MG132 for indicated lengths of time. (C) HEK293T cells were transfected with indicated vectors and treated with MG132. Cell lysates were immunoprecipitated with anti-FLAG (NIK) antibody. Ubiquitinated and total NIK was detected by immunoblot analysis of anti-HA and anti-FLAG antibodies, respectively (upper). Immunoblot analysis of SOCS3, FLAG (NIK), HA (Ub), and GAPDH in whole-cell lysates (WCL) (lower). (D) Flow-cytometry analysis of iPCs among WT and TKO iGCB cells transduced with empty retroviruses (Vector) or retroviruses encoding NIK, IRF4, and Blimp1 (upper). Summary of the percentage of iPCs (lower). Small horizontal lines indicate the means (±SEM). *p < 0.05, ***p < 0.001. Data are representative of three independent experiments. See also Figure S9.

Article Snippet: Anti-IgG1 or anti-NP IgG1 spots were detected by biotin-conjugated anti-mouse IgG1 antibody (Southern Biotechnology, 1070-08) in combination with Av-HRP and AEC substrate (Vector Laboratories, A-2004& SK-4200).

Techniques: Ubiquitin Proteomics, Cell Culture, Immunoprecipitation, Western Blot, Transfection, Flow Cytometry, Transduction, Plasmid Preparation